The nanoString nCounter® SPRINT profiler integrates sample preparation and analysis into a single instrument, allowing the analysis of up to 800 RNA targets simultaneously using single-tube multiplexing. It can detect both RNA and DNA from a variety of sample types, including tissue (either fresh, frozen or FFPE preserved). The nanoString nCounter technology additionally benefits from low input requirements (50-150 ng RNA and 5-300 ng DNA) without the need for reverse transcription or amplification.
Nucleic acid targets are directly labelled through hybridisation with single target-specific molecular barcodes (colour-coded probes).
Probe-nucleic acid hybridisations are purified, immobilised and aligned on a nCounter cartridge and the barcodes counted.
The probe set panels can either be pre-designed or custom-made using nanoString’s design service.
Data from the nCounter SPRINT Profiler can be directly imported into the freely available nSolver software for analysis.
For further information about the nanoString nCounter® SPRINT profiler please consult: https://nanostring.com/products/ncounter-analysis-system/sprint-profiler/
For queries relating to collaborating with the Genomics teams at The University of Sheffield please email: genomics-group@sheffield.ac.uk