Sample QC

A range of services for the accurate quantitation and quality assessment of nucleic acids – an important precursor to downstream analyses such as real-time PCR or Sequencing

TapeStation 4200 Bioanalyzer 2100 Femtopulse
  • An automated gel electrophoresis platform that qualifies and quantifies nucleic acid samples to aid next generation sequencing (NGS) workflows.
  • Uses 1-2µl of sample
  • Up to 96 samples per run
  • A chip-based capillary electrophoresis platform that provides quality, size and integrated assessments for DNA and RNA, as well as quantification information
  • Uses 1-2µl of sample
  • Up to 11 samples per run>
  • A parallel capillary electrophoresis toll for separating, sizing and quantifying low concentration, high molecular weight DNA fragments and smears
  • Uses 1µl of sample
  • Up to 95 samples per run
  • Qubit/Qubit Flex Synergy LX Flourometer NanoDrop 8000
  • A benchtop flurometer designed for quantifying DNA and RNA
  • Broad range and high sensitivity DNA/RNA concentration assays available
  • Uses 1µl of sample
  • Between 1-8 samples per run
  • A benchtop flourometer designed for quantifying DNA
  • Designed for Quantiflor dsDNA System assays
  • Uses 1.5µl of sample
  • Up to 88 samples per run
  • A UV-Vis Spectrophotometer designed for measuring DNA/RNA/Protein concentration and purity (260:280, 260:230)
  • Uses 1-2µl of sample
  • Eight samples per run

  • For queries relating to collaborating with the Genomics teams at The University of Sheffield please email: genomics-group@sheffield.ac.uk